Mwp 22

MWP Introduction


The Zantiks MWP unit helps standardise behavioural phenotyping in model organisms, such as larval zebrafish, Drosophila and Daphnia. To use the MWP unit you need a small space on your lab bench, a nearby power supply, and your own browser-enabled device (smartphone, table, laptop, PC, Mac).

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Zantiks MWP kit

  • Integrated unit (approx. 270mm H; 220mm D; 160mm W) with:
    • Zanscript (Zantiks proprietary scripting software)
    • Built-in features / stimuli
      • overhead 'house lights'
      • screen on the MWP_sc
      • vibration motor on the MWP_vb
      • Camera
      • Computer
  • A 12V power cable
  • Zantiks router, power cable, and Ethernet cable
    • Zantiks router is supplied with the first unit, this is typically a Cisco router with 4 LAN ports and 1 WAN port. Subsequent units can be networked to this router, using a network switch if necessary.
  • Fits standard multiwell plates and Petri dishes (not supplied)
  • A temperature control system is available for both MWP units which is currently a free add-on for users
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Behavioural studies in the MWP include:

  • Locomotor activity
  • Circadian rhythm monitoring
  • Toxicity, phenotyping and drug screening
  • Startle response, habituation and prepulse inhibition
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Assembling the motor plate

If you have purchased the MWP_vb unit you need to insert the motor plate into the unit before using it. The motor plate is delivered detached in order to prevent breakages in transit.

The video below shows how to slide up the front section of the MWP_vb unit so that the motor plate can be inserted and attached. You will need a T10 Torx driver, or similar, not an allen key to do this (this tool should be included with your unit).

Please note the MWP_sc unit, used with Drosophila, does not have a built-in motor, so these assembly instructions are not valid for this version.

How to assemble the motor plate to the MWP_vb unit

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Setting up the MWP

How to set up the MWP unit: including the layout of the cables in the socket panel and how to insert a wellplate into the chamber, with or without a stand.

  1. Place the MWP unit on a stable surface close to a power socket and away from disturbance.
  2. Insert the 12V power supply cable into the top left of side socket of the MWP and plug it into the mains socket (see image below).
  3. Connect the ethernet cable at the side of the MWP unit, just below the 12V power cable, and plug this into any of the 4 slots available in the LAN section of the router.
  4. Turn on the router and turn on the mains socket for the Zantiks unit. Wait a few minutes for the MWP unit to power on. When using the MWP version with a screen text scrolls across the screen before displaying the IP address of the unit (which begins with the same 7 digits 192.168.1.). When using the MWP_vb, your IP address is included on your delivery note.
    1. If you have only one Zantiks unit, the address will be 192.168.1.101. The IP address is used to identify the unit on the Zantiks Control Console.
  5. Using any browser-enabled device:
    1. go to your device’s network settings and connect to the wifi network “Zantiks”
    2. type in the network password, which is initially set up as: behaviour, simply
    3. go to the address bar of the web browser and type in the IP address for your Zantiks unit (see step 4).
  6. Your web browser should now display the Zantiks Control Console page.
Img 0042
Side of MWP unit showing where to insert the green connector plugs and cables. Bottom left the Ethernet cable plugs in; directly above it is the 12V power supply cable. On the bottom board are two 2-way green, connector plugs. On the top green connection board are the 4-way connector plug for the thermistor (left) and the 4-wire connector plug for the overhead lights (right).
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Temperature control for MWP

The MWP unit comes with a temperature control add-on which you can attach to the unit. This add on enables you to set the temperature in the experimental chamber to any temperature within 10°C above and 2°C below the room's ambient temperature.

The video below demonstrates how to attach the temperature control add-on to the unit. The add-on consists of 2 insulated tubes, a black fan motor and 16 screws. There is also an allen key which fits the screws; tighten each screw securely so no air leaks between the fan motor, the MWP unit and the insulated tubes.

Please note:

In some cases there may be condensation created in the fan motor during use, in which case the water will come out under the front end of the fan motor unit.

The fan will turn on as soon as the 2-wire 3 way green connector plug is inserted into the MWP socket, as long as the MWP unit is powered.

To operate the temperature control unit please refer to the Temperature control MWP demo script in the website.

How to attach the temperature control add on to the MWP unit

Next, go to the manual, Zantiks Control Console, to see how you operate the unit via your web browser.