Zantiks MWP


The MWP units are small, highly capable systems for tracking and providing stimuli for small organisms in multi well plates, petri dishes or custom chambers, such as larval zebrafish, adult and larval Drosophila, and neonate to adults Daphnia.

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Benefits

All the Zantiks units are built along the same principles, enabling high throughput and easy-to-run experiments. The benefits of choosing Zantiks include:

  • An isolated and stable testing environment
  • Networked and controlled via a web browser
  • Live video view and real-time video recording
  • Includes software, uses the Zanscript language for experiment specification
  • Real-time results processing and output in .csv format
  • Integrated camera, computer, experimental chamber and built-in stimuli
  • Takes up little bench space
  • Only requires 12V power and a network connection
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Zantiks MWP unit with testing chamber that fits a standard well plate
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Target Studies

The MWP systems can achieve a range of studies, on larval zebrafish, Drosophila and Daphnia and other similarly sized organisms, including:

  • locomotor activity
  • toxicity
  • startle response
  • habituation
  • prepulse inhibition
  • circadian rhythm

Please contact us if you have any specific studies in mind.

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Zantiks MWP kit

The MWP system has an overall unit size of (approx. mm): 190 width, 230 length, 480 height and is fully integrated with:

  • Zantiks proprietary software
  • Computer
  • Camera
  • Overhead lights
  • Temperature monitoring
  • A vibration plate
  • A light stand
  • Standard temperature control


The MWP fits standard multi-well plates and Petri dishes (not supplied). Tailored inserts for specific studies can be ordered direct from Zantiks.

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Built-in stimuli

  • Overhead illumination – colour, brightness and duration can be specified with ms accuracy.
    • Can be used for ambient lighting, circadian rhythm entrainment and light flashes.
  • Vibratory stimulus – duration, intensity and frequency can be precisely controlled with ms accuracy.
    • Suitable for generating startle responses and for PPI.
  • Bright light stimulus - available in a range of wavelengths. Duration, Lux and frequency can be precisely controlled with ms accuracy.
    • Suitable for optogenetics, startle responses and PPI.
  • Temperature control - allows raising the temperature above that of ambient room temperature.
    • Suitable for maintaining favourable temperatures or for heat shock.
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Included equipment

  • Light stand
    • Plugs directly into the MWP units - no additional power supply needed.
    • Control both the brightness and duration of the stimulus through Zanscript.
    • Currently available with peak stimulation at 470nm.
    • Available in different wavelengths, please contact us to discuss your requirements.

Note: we have confirmed the plate can stimulate zebrafish larvae, and adult Drosophila but we recommend full evaluation of stimulation performance in different wells.

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Light stand placed inside the testing chamber of the MWP (shown without the foam insulating door for visibility).
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View of the light stand from the side with light on.

  • Standard temperature control unit
    • Connects to side of the MWP units.
    • Control of the temperature up to 10°C above ambient.
    • Operates via moving air through the testing chamber.
Both Tc From Side
Right: the standard temperature control unit is shown attached to the right side of the system. Left: the peltier temperature control unit is seen attached on both sides of the system.
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Add-on equipment

  • Peltier temperature control unit
    • Allows for a greater range of control of air temperature compared to the standard temperature control system.
    • A separate unit that easily connects to both the MWP units.
    • Control of the temperature from 8°C below to 12°C above ambient.
    • Operates via circulating the air in the testing chamber.
Temp Control
Adjusting the temperature in the MWP unit above and below ambient room temperature
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Temperature control options for the MWP

The Zantiks MWP units provide an enclosed and isolated environment for behavioural research. However, one of the consequences of the enclosed environment, and the stimuli used, is that the temperature within can rise above ambient.

To combat this, Zantiks have two options for enabling temperature control within its MWP units. Both systems attach to the side of the unit, and move air through the chamber in which the animals are placed. Both systems are powered entirely from the MWP unit, and both can be controlled from the Zanscript experimental scripting language.

As both systems operate by moving air through the unit, it may be beneficial for some experiments (such as long-term or animals that require humidity) to use lids or covers on the multi-well plates / petri dishes to reduce evaporation from the chambers containing animals.

Both Tc From Side
Side view of both temperature control systems. The MWP on the left has the peltier system and the unit on the right has the standard system
Both Tc From Above
Aerial view of both temperature control systems. The MWP on the left has the peltier system and the unit on the right has the standard system. Note that about 9 x the area is required for the peltier system

Standard Temperature Control System (included with purchase of the MWP unit)

This system moves ambient air through the unit slowly. If/when required, the air can be heated to achieve a temperature above that of the ambient lab temperature by up to 10°C.

This system allows one to achieve standard conditions that are normally required for experiments. For example, for Drosophila experiments, in an air conditioned lab of about 20°C, you can achieve the standard 22°C or 25°C and for zebrafish larvae experiments, in a room at approximately 25°C, you could achieve the standard 28°C.

Bench area required - 30 cm x 30 cm


Peltier Temperature Control System

This system is much larger, and considerably increases the bench space needed to operate the MWP unit. However, it does allow for a greater range of air temperature control away from the ambient. It will enable control of 6°C below the ambient laboratory temperature and 12°C above (with scope for up to 8°C below and 15°C ambient laboratory temperature).

Bench area required - 90 cm x 90 cm

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How to operate

Fully networked, Zantiks units are supplied with a router and controlled via a web browser, allowing users to operate them from their own phone, tablet, laptop, PC, Mac or Chromebook.

From the web browser on your device you can edit scripts, start and stop chosen services, view live video, download results (.csv files).

To operate a Zantiks unit connect to the same network as the unit. To open the Zantiks Control Console, enter the unit's IP address in the browser (supplied on your delivery note). From this home page you can operate all your networked units.

For instructions on how to set up the MWP or any add on's, see the MWP Introduction manual.

For details on how to use the Zantiks Control Console, see the Zantiks Control Console manual.

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Tracking and video recording

Tracking of up to 96 single-housed small animals can be carried out at 30 Hz in the MWP. Video recordings may be made simultaneously.

Tracking larval zebrafish in a 24-well plate in the Zantiks MWP

Tracking adult Drosophila in the Zantiks MWP