The MWP units come with a Standard temperature control system which is able to increase and hold the temperature up to 10°C above current ambient room temperature.
Alternatively, the Peltier temperature control system, which has a temperature control range of 8°C below to 12°C above ambient room temperature, is offered as an optional add-on.
The time taken to change the chamber's temperature will vary according to what is in the chamber (e.g., a well plate), but this is typically completed within 30 minutes.
A thermistor is located on the left hand side of the MWP unit, just in front of the side panel in the experimental chamber. The current temperature recorded by the thermistor is reported on the unit's Zantiks Control Console and can be written on to a data file at any time within a script.
For help connecting either of the temperature control systems to the MWP unit, please refer to the 'Temperature control' section found in the MWP Introduction manual.
How to set the temperature
Set up the experimental chamber with the equipment required for your experiment (e.g., the diffuser stand or light stimulation plate, and a multi-well plate or petri dish).
Then run the script below. The Zantiks unit will adjust the temperature until it reaches the temperature set by the thermostat, as recorded on the Zantiks Control Console. The temperature will be maintained at this level within ±0.5°C.
Note: the thermostat setting will be retained and used even if the script is not running. No thermostat will be set when the unit is first turned on.
ACTION MAIN #SET(TCS_MODE,PELTIER) # note: you must remove the hash to include this line if using the peltier control system SET(THERMOSTAT,20) WAIT(1) COMPLETE
The script above tells the temperature control unit to set the thermostat at 20°C and adjusts the temperature in the experimental chamber accordingly (this may take up to 10-15 minutes depending on the difference between the temperature set and the current temperature within the chamber).
Once the chamber has reached the desired temperature, you are ready to add your animals.
Please note: If you are using software from before mid-2020, and have a peltier system, DO NOT remove the # from the script above. The older software will not recognise this line of code.
How to record temperature in results data
If you would like to record the temperature in your processed results data file, you can adapt the code in the script below.
DEFINE NUMBER_OF_BINS 30000000 DEFINE BIN_TIME_SECS 10 #SET(TCS_MODE, PELTIER) SET(THERMOSTAT,20) ACTION MAIN WAIT(1) LOGCREATE("TEXT:TIME|TEXT:INLET_TEMP") LOGRUN() INVOKE(MTEMP,NUMBER_OF_BINS) COMPLETE ACTION MTEMP WAIT(BIN_TIME_SECS) LOGCREATE("RUNTIME|TEMPERATURE1") LOGAPPEND("TEMPERATURE2|TEMPERATURE3|TEMPERATURE4") LOGRUN() COMPLETE
In the above demo script:
The SET(THERMOSTAT,#) command sets the temperature in the chamber at 20°C (this must be within the ambient room temperature and 10°C above).
The LOGCREATE command details the labels for the cell data in a line of cells (i.e., the text labels 'TIME' in cell 1 and 'INLET_TEMP' in cell 2 for columns of data). The following LOGRUN() line tells the unit to output the previous LOGCREATE line.
LOGCREATE("RUNTIME|TEMPERATURE1") LOGAPPEND("TEMPERATURE2|TEMPERATURE3|TEMPERATURE4") LOGRUN()
The LOGCREATE (and LOGRUN) command above tells the unit to output in a line of cells the actual runtime at that point in the experiment in cell 1, and the temperature recorded by the thermistor in cell 2, 3, 4 and 5.
Try running this demo script in your MWP unit and take a look at the data output.
To download the set_record_temp_MWP demo script as a .zs file (file type Zantiks software reads), choose the Save File As option in the right-click dialogue box. Clicking on the script name hyperlink will open a read-only version of the script.