The Zantiks AD helps standardise behavioural experiments in animal models such as adult zebrafish and mice. To use the Zantiks unit you need a small space on your lab bench, a nearby power supply, and your own browser-enabled device (e.g. smartphone/tablet/ laptop/PC/ Mac).
Zantiks AD kit
- Integrated unit (approx. 500mm H; 300mm D; 220mm W) with:
- Tank / cage
- Built in stimuli (overhead ‘house lights’, screen, mild electric shock, solid and liquid delivery)
- Zanscript (Zantiks software)
- One tank (approx. 150mm H; 200mm D; 140mm W)
- Tank inserts to create multiple different arenas:
- Arena lengthwise dividing inserts x 3
- Arena widthwise dividing inserts x 3
- Food magazine insert x 1
- 5-aperture insert x 1
- 2 metal shocking plates with cable and testing LED strip
- Peristaltic pump unit (we recommend you use the following tubing with it: 2mm internal diameter, 4mm external diameter silicone tubing)
- A 12V power cable
- Zantiks router* and power cable
- Ethernet cable
*One Zantiks router is supplied with the first unit, this is usually a Cisco router with 4 LAN ports and 1 WAN port. Subsequent units can be networked to this router, using a network switch if necessary.
Behavioural studies in the AD include:
- 5-choice serial reaction time task (5-CSRTT)
- Matching to sample
- Reversal learning
- Aversion learning
- Startle response, habituation and prepulse inhibition
- Conditioned place preference
Setting up the AD
- Place the AD unit on a stable surface close to a power socket and away from disturbance.
- Insert the 12V power supply cable into the back socket of the AD and plug it into the mains socket.
- Connect the ethernet cable at the back of the Zantiks unit and plug this into any of the 4 slots available in the LAN section of the router (see image below).
- Turn on the router and turn on the mains socket for the Zantiks unit. When the Zantiks unit powers on, a text screen will load and scroll across the screen in the unit.
- The unit’s IP address will be displayed on the screen each time the unit is turned on. The IP address is used to identify the unit on the Zantiks Control Console.
- Using any browser-enabled device (smartphone/tablet/laptop/PC/Mac):
- go to your device’s network settings and connect to the wifi network “Zantiks”
- type in the network password, which is initially set up as: behaviour, simply
- go to the address bar of the web browser and type in the IP address for your Zantiks unit (as mentioned above).
- Your web browser should now display the Zantiks Control Console page.
Setting up the side screen
The AD unit comes with a built-in bottom screen and the option to buy a side screen for lateral presentation. The side screen can be used instead of the bottom screen, both screens can not be in use at the same time.
The side screen unit either sits at the back or the front of the chamber. It comes with an extension HDMI cable (to use when the screen sits at the front of the chamber), a tank (with black sides apart from a clear bottom and one clear end wall) and a 2-choice insert. We are also developing a 2-choice feeder insert which can be used when image and solid food stimuli are combined in the same location at the front of the chamber.
- The AD unit can only have one screen plugged in at a time.
- When switching over the bottom screen to the top screen you need to shutdown the unit and unplug its power supply.
- The bottom screen has 2 cables which come out from the base of the unit’s back - an HDMI (thick black) cable which plugs into the top box and a black 2-wire cable with a 2-plug green connector which either plugs in to CN1 or CN2. (N.b. the other black and red 2-wire cable with 2-plug green connector is for the LED lights at the base of the unit.)
- Unplug the bottom screen’s 2 cables with care.
To insert the side screen into the back of the unit thread the 2 cables through the circular hole at the back of the unit and slide the screen back until it is flush against the back wall, and the two screws on the bottom screen fit neatly into the two round slots on the bottom of the screen unit.
Push the side screen in to the back of the unit so that it is flush against the back wall, with the cables fully out of the unit. The two screws on the bottom screen fit neatly into the two round slots on the bottom of the side screen unit.
- Once the side screen is in place, plug in the side screen cables as shown in the image above (Side screen cables), taking care to insert the HDMI cable in the slot found in the back of the top box, and the 2-way green plug into CN1 (or CN2).
- Re-connect the unit to the power supply.
The external lights
An external light strip can be used with the side screen to help use top lights to draw attention to the feeder when it is activated.
The external light strip's 4-way green plug connects into the back of the top box at CN7.
The external light strip hooks on to the top of the end of tank, once the tank is in the chamber. We recommend that you first try attaching the light strip to the tank whilst the tank is out of the chamber, then take it off when you slot the tank into the chamber (as the tank will not slide into the chamber with the light strip attached).
How to use the side screen
The side screen has different dimensions to the bottom screen, and images that are on the side screen will appear elongated (as the pixels are not square, but instead 1 x 0.7). Consequently you need to resize any images / bitmaps that you use so that the horizontal dimension is 0.7 of the original bitmap.
For details on how to write a script and create bitmap images for the side screen see the Side screen demo script webpage.