Zantiks MWP


The MWP units are small highly capable systems for tracking and providing stimuli for small organisms in multi well plates, petri dishes or custom chambers, such as larval zebrafish, adult and larval Drosophila, and neonate to adults Daphnia.

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Benefits

All the Zantiks units are built along the same principles, enabling high throughput and easy-to-run experiments. The benefits of choosing Zantiks include:

  • An isolated and stable testing environment
  • Networked and controlled via a web browser
  • Live video view and real-time video recording
  • Includes software, uses the Zanscript language for experiment specification
  • Real-time results processing and output in .csv format
  • Integrated camera, computer, experimental chamber and built-in stimuli
  • Takes up little bench space
  • Only requires 12V power and a network connection
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Zantiks MWP unit with testing chamber that fits a standard well plate
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Target Studies

The MWP systems can achieve a range of studies, on larval zebrafish, Drosophila and Daphnia and other similarly sized organisms, including:

  • locomotor activity
  • toxicity
  • startle response
  • habituation
  • prepulse inhibition
  • circadian rhythm

Please contact us if you have any specific studies in mind.

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Zantiks MWP kit

The two versions of the MWP systems' external structure are identical, with an overall unit size of (approx. mm): 190 width, 230 length, 480 height


MWP_vb

Equipped with a vibration plate for delivering vibratory/auditory stimuli. This version is recommended for tracking larval zebrafish, Daphnia and other animals in water.

MWP_sc

Equipped with a built-in screen at the bottom of the tracking chamber. This version is recommended for tracking Drosophila.


Inserts

The MWPs fit standard multiwell plates and Petri dishes (not supplied). Tailored inserts for specific studies can be ordered direct from Zantiks.

MWP Technical specification

Integrated units MWP_vb MWP_sc
Computer
Camera
Overhead lights
Temperature monitoring
Vibration
Screen
Optogenetic add-on compatible
Temperature control add-on compatible
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Built-in stimuli

  • Overhead illumination – colour, brightness and duration can be specified with ms accuracy.
    • Can be used for ambient lighting, circadian rhythm entrainment and light flashes.
  • Temperature monitoring - measured from a thermistor mounted at the side of the testing chamber. The current temperature is displayed on the home screen of the console.
  • MWP_vb vibratory stimulus – duration, intensity and frequency can be precisely controlled with ms accuracy.
    • Suitable for generating startle responses and for PPI studies.
  • MWP_sc screen to display images - display colours and images
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Included equipment

  • Temperature control unit
    • A separate unit that easily connects to both the MWP units.
    • Control of the temperature to -2°C to + 10°C from ambient.
    • Operates via circulating the air in the testing chamber.
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Add-on equipment

Optogenetic insert

  • Plugs directly into the MWP units - no additional power supply needed.
  • Control both the brightness and duration of the stimulus through Zanscript.
  • Currently available with peak stimulation at 470nm. We can build and supply for different wavelengths, please contact us to discuss your requirements.

Note: we have confirmed the plate can stimulate zebrafish larvae, but we recommend full evaluation of stimulation performance in different wells.

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Optogenetic module placed inside the testing chamber of the MWP (shown without the foam insulating door for visibility).
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View of the optogenetic insert from the side with light on.
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How to operate

Fully networked, Zantiks units are supplied with a router and controlled via a web browser, allowing users to operate them from their own phone, tablet, laptop, PC, Mac or Chromebook.

From the web browser on your device you can edit scripts, start and stop chosen services, view live video, download results (.csv files).

To operate a Zantiks unit connect to the same network as the unit. To open the Zantiks Control Console, enter the unit's IP address in the browser (supplied on your delivery note). From this home page you can operate all your networked units.

For instructions on how to set up the MWP, see the MWP Introduction manual.

For details on how to use the Zantiks Control Console, see the Zantiks Control Console manual.

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Tracking and video recording

Tracking of up to 96 single-housed small animals can be carried out at 30 Hz in the MWP. Video recordings may be made simultaneously.

Tracking larval zebrafish in a 24-well plate in the Zantiks MWP_vb

Tracking adult Drosophila in the Zantiks MWP_sc