The effects of an obesogenic diet on behaviour and cognition in zebrafish (Danio rerio): traits average, variability, repeatability, and behavioural syndromes (2022)


Anwer, H., O'Dea, R. E., Mason, D., Zajitschek, S., Klinke, A., Reid, M., Hesselson, D., Noble, D.W.A., Morris, M.J., Lagisz, M., & Nakagawa, S. (2022, February 7). The effects of an obesogenic diet on behaviour and cognition in zebrafish (Danio rerio): traits average, variability, repeatability, and behavioural syndromes. Retrieved from ecoevorxiv.org/ewq64

doi: 10.32942/osf.io/ewq64

Abstract

The obesity epidemic, largely driven by the accessibility of ultra-processed high-energy foods, is one of the most pressing public health challenges of the 21st century. Consequently, there is increasing concern about the impacts of diet-induced obesity on behaviour and cognition. While research on this matter continues, to date, no study has explicitly investigated the effect of obesogenic diet on variance and covariance (correlation) in behavioural traits. Here, we examined how an obesogenic versus control diet impacts means and (co-)variances of traits associated with body condition, behaviour, and cognition in a laboratory population of ~160 adult zebrafish (Danio rerio). Overall, an obesogenic diet increased variation in several zebrafish traits. Zebrafish on an obesogenic diet were significantly heavier and displayed higher body weight variability; fasting blood glucose levels were similar between control and treatment zebrafish. During behavioural assays, zebrafish on the obesogenic diet displayed more exploratory behaviour and were less reactive to video stimuli with conspecifics during a personality test, but these significant differences were sex-specific. Zebrafish on an obesogenic diet also displayed repeatable responses in aversive learning tests whereas control zebrafish did not, suggesting an obesogenic diet resulted in more consistent, yet impaired, behavioural responses. Where behavioural syndromes existed (inter-class correlations between personality traits), they did not differ between obesogenic and control zebrafish groups. By integrating a multifaceted, holistic approach that incorporates components of (co-)variances, future studies will greatly benefit by quantifying neglected dimensions of obesogenic diets on behavioural changes.

Keywords

obesogenic diet; zebrafish; variance; personality; anxiety; cognition; high fat diet