A locomotion-based assay to measure prepulse inhibition in zebrafish larvae (2024)

Read, E. & Hindges, R. (2024). A novel locomotion-based prepulse inhibition assay in zebrafish larvae.
microPublication Biology, 10.17912/micropub.biology.000914.

doi: 10.17912/micropub.biology.000914


Sensory gating, measured using prepulse inhibition (PPI), is an endophenotype of neuropsychiatric disorders that can be assessed in larval zebrafish models. However, current PPI assays require high-speed cameras to capture rapid c-bend startle behaviours of the larvae. In this study, we designed and employed a PPI paradigm that uses locomotion as a read-out of zebrafish larval startle responses. PPI percentage was measured at a maximum of 87% and strongly reduced upon administration of the NMDA receptor antagonist, MK-801. This work provides the foundation for simpler and more accessible PPI assays using larval zebrafish to model key endophenotypes of neurodevelopmental disorders.