There is the facility to measure the temperature in the experimental chamber in the MWP unit. You can also use the temperature control add on to change and hold the temperature between 10 degrees above and 2 degrees below the room's ambient temperature. The time taken to change the chamber's temperature will vary according to what is in the chamber, but this is normally completed within 30 minutes.
A thermistor is located on the left hand side of the MWP unit, just infront of the side panel in the experimental chamber. The current temperature recorded by the thermistor is reported on the unit's Zantiks Control Console and can be reported at any time in a script.
To attach the temperature control add on to the MWP unit please refer to the video in the 'Temperature control' section found in the MWP Introduction manual.
How to set the temperature
Set up the experimental chamber with the equipment that you will be using during your experiment, eg with the diffuser stand or with the optogenetics stand, and a multiwell plate.
Then, run the following script. The Zantiks unit will then adjust the temperature until the temperature recorded on the Zantiks Control Console reaches the temperature set by the thermostat. The temperature will then be maintained at this level within 0.5 degrees.
Please note the thermostat setting will be retained and used even if the script is not running. No thermostat will be set when the unit is first turned on.
INCLUDE zsys ACTION MAIN SET(THERMOSTAT,35) WAIT(1) COMPLETE
The script above will tell the temperature control add on to set the thermostat at 35 degrees, it will then adjust the temperature in the experimental chamber to this temperature (which may take up to about 10/15 minutes depending on how much it needs to change and what is in the chamber).
Once the chamber has reached the desired temperature, set up the experimental chamber with a multiwell plate with your animals.
How to record temperature in results data
If you would like to record the temperature in your processed results data file, you need to adapt the code in the script below for your own script.
INCLUDE zsys DEFINE NUMBER_OF_BINS 30000000 DEFINE BIN_TIME_SECS 10 SET(THERMOSTAT,35) ACTION MAIN WAIT(1) LOGCREATE("TEXT:TIME|TEXT:INLET_TEMP") LOGRUN() INVOKE(MTEMP,NUMBER_OF_BINS) COMPLETE ACTION MTEMP WAIT(BIN_TIME_SECS) LOGCREATE("RUNTIME|TEMPERATURE1") LOGAPPEND("TEMPERATURE2|TEMPERATURE3|TEMPERATURE4") LOGRUN() COMPLETE
In the above demo script:
The line of code above sets the temperature in the chamber at 35 degrees (which must be between 10 degrees above and 2 degrees below ambient temperature outside the unit).
The LOGCREATE command details the labels for the cell data in a line of cells, i.e. the text labels 'TIME' in cell 1 and 'INLET_TEMP' in cell 2. The following LOGRUN() line tells the unit to output the previous LOGCREATE line.
LOGCREATE("RUNTIME|TEMPERATURE1") LOGAPPEND("TEMPERATURE2|TEMPERATURE3|TEMPERATURE4") LOGRUN()
The LOGCREATE (and LOGRUN) command above tells the unit to output in a line of cells the actual runtime at that point in the experiment in cell 1, and the temperature recorded by the thermistor in cell 2, 3, 4 and 5.
The normal way might be to record the temperature in every line of results data.
To download the set_record_temp_MWP demo script as a .zs file (file type Zantiks software reads), choose the Save File As option in the right-click dialogue box. Clicking on the script name hyperlink will open a read-only version of the script.
Script download: set_record_temp_MWP.zs