5-Choice serial reaction time task (5-CSRTT) (zebrafish)

The 5-choice serial reaction time task, 5-CSRTT, is a continuous performance test used to assess attentional processes and impulse control.

During this task, an animal is required to respond to the presence of a briefly presented stimulus (usually a light) in one of 5 locations at the rear of the testing chamber. Aspects of attention and executive performance can be investigated through manipulation of stimulus light exposure length and the addition of distractors. While, impulsivity can be measured through the animals’ premature or anticipatory responding (i.e., responding prior to the stimulus light onset).


Experimental setup

The Zantiks AD unit is used for the 5-CSRTT in zebrafish. The five aperture and food hopper inserts are used to create the experimental environment (see image A below). The food hopper insert is designed to form an area for the fish to enter and collect food reward, without the food escaping into the remainder of the tank, as well as an entry point for initiating trials. The five aperture insert creates five distant entry points at the opposite end of the tank from food delivery. The five holes act in a similar fashion to the five nose poke holes in a rodent version of the task.

White light is typically used as stimuli for this experiment, which is presented from the integrated screen below the testing tank. Target zones are assigned to specific locations within the tank (e.g., the five stimulus light locations, see image B below). Responses are detected when a fish enters these zones (see image C below).

The 5-Choice serial reaction time task inserts in the Zantiks AD unit:

Ad Tank Hop 5Ap
A) This is an overhead view of the inserts in a testing tank. The five apertures can be seen inserted to the right of the tank. The food-reward delivery zone is seen inserted to the left of the tank.
5 Choice Detector Zones in Zantiks AD
B) A schema of the typical target zones for the 5-CSRTT. Stimuli are presented in an initiator zone (red square), feeding area (orange rectangle) and five apertures at the other end of the tank (yellow, green, blue, magenta, and cyan squares). Note that colours do not represent the stimulus light colour used in the task.

C) Video recorded during the running of the experiment. A fish is tracked, as indicated by the white cross. Stimuli are not visible on the live tracking image due to the infra-red tracking, so the stimuli are denoted on the image with coloured boxes.


Experimental procedure

5-CSRTT training is divided into five distinct training stages.

  1. Habituation/Autoshaping: Subjects are concurrently acclimated to the testing tank and autoshaped to the food hopper. Individual fish are placed in a Zantiks AD tank and shaped to swim into the food delivery area when the hopper light is illuminated. Following a 60 sec intertrial interval (ITI) the food hopper light illuminates and food is delivered. This step allows the fish to habituate to the noise of food delivery and to the testing environment, as well as learn to enter the food delivery area.
  2. Stimulus Light Training: Fish are next trained to trigger any of the five stimulus lights as a method of orientation to the stimuli. All five stimulus lights are simultaneously illuminated in the five apertures at the back of the tank. The fish needs to enter any of the apertures in order for the food hopper light to illuminate and food reward to be delivered. If they do not respond in the limited hold (LH; 30 sec), no food reward is delivered. A new trial begins following a 20 sec ITI.
  3. Initiator Light Training: Fish learn to trigger the initiator light (illuminated for 30 secs) to begin a trial. Triggering the initiator light, illuminates the food hopper light and food reward delivery. This is followed by the fish learning to trigger the initiator light (beginning the trial), followed by triggering any of the five illuminated stimulus lights at the end of the tank to receive food reward.
  4. Stimulus Light Discrimination: The fish now initiates the trial, which illuminates a single stimulus light. Stimulus lights are presented in random order. The fish must enter the illuminated aperture during the LH to receive the food reward.
  5. 5-CSRTT: The final step is conducted similarly to the 4th, with the addition to a n-sec delay. The fish initiates the trial, and needs to withhold from responding during the set ITI. One of the five apertures is then briefly illuminated and the fish must now respond within the LH in order to receive food reward.

Results/data output

There are several parameters that are commonly used in the 5-choice serial reaction time tasks including: correct responses (responses to correct stimulus light) and incorrect responses (responses to the incorrect location), omissions (not responding following stimulus presentation) and premature responses (responding to any of the apertures prior to stimulus presentation). Zantiks AD units can be scripted to automatically process these variables.

  • Accuracy: CORRECT/(CORRECT + INCORRECT)*100 – a measure of selective attention
  • Percent Correct: CORRECT / (TOTAL NUMBER OF TRIALS)*100 – a measure of overall performance
  • Percent Omission: OMISSIONS / (OMISSIONS + CORRECT + INCORRECT)*100 – a measure of sustained attention/motivation
  • Anticipatory responses: EARLY/(EARLY + CORRECT + INCORRECT)*100 – a measure of impulsivity/response inhibition

The number of sessions needed to reach criterion for a stage of training in the 5-CSRTT can be analysed by repeated measures ANOVA (GLM-RM) with group (e.g., drug dose, genotype) (between) and session (within) factors.

5 Csrtt Results
Representative data from 5-CSRTT training and testing. A: Initiator Light Training. Fish trigger the initiator light. Criterion met after 6 training days [GLM: F6,36 = 40.39, p < 0.001]. B: Initiator Light Training. Fish trigger the initiator light before trigger 1 of the 5 light apertures. Criterion reached in 4 training days [GLM: F3,21 = 16.58, p < 0.001]. C: Stimulus Light Discrimination. Fish must trigger the initiator light before then triggering the singly presented stimulus light (randomly presented). Criterion reached in 10 days [GLM: F8,48 = 6.44, p < 0.001]. Data from Brock et al., 2017.


Brock, A.J., Sudwarts, A., Daggett, J.M., Parker, M.O., Brennan, C.H. (2017). A fully automated computer-based ‘Skinner Box’ for testing learning and memory in zebrafish. bioRxiv 110478.
Parker, M.O., Millington, M.E., Combe, F.J., & Brennan, C.H. (2012). Development and implementation of a three-choice serial reaction time task for zebrafish (Danio rerio). Behavioural Brain Research, 227(1), 73–80.
Parker, M.O., Brock, A.J., Sudwarts, A., & Brennan, C.H. (2014). Atomoxetine reduces anticipatory responding in a 5-choice serial reaction time task for adult zebrafish. Psychopharmacology, 231(13), 2671-2679.
Parker, M.O., Ife, D., Ma, J., Pancholi, M., Straw, C., Smeraldi, F., & Brennan, C.H. (2013). Development and automation of a test of impulse control in zebrafish. Frontiers in Systems Neuroscience, 7, 65.