Zantiks LT


The Zantiks LT unit is designed to measure the behaviour of rodents and fish. Similar in functionality to the AD unit, but double the size, the LT unit is suited to studies with rodents and larger fish, such as guppies.

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Benefits

Zantiks units are increasingly popular with labs worldwide. All the different types of unit are built along the same principles, enabling high throughput and easy-to-run experiments. The benefits of choosing Zantiks are that each unit:

  • Provides an isolated and stable environment for consistent results
  • Is networked and controlled via the web browser of any device
  • Generates live video view, provides real-time video recording
  • Uses the Zanscript language for experiment specification
  • Provides real-time results processing and output in .csv format
  • Has an integrated camera, computer, experimental chamber and built-in stimuli
  • Requires only 12V power and a network connection


Zantiks_LT_unit
Zantiks LT unit
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Target studies for rodents

The Zantiks LT is suitable for:

  • Tracking rodents / fish in experimental environments (e.g., open field, Y-maze, Conditioned place preference).
  • Training rodents and fish with food reward in operant tasks such as discrimination, 5-choice, match to sample.
  • Tracking multiple animals singly-housed in individual arenas.
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Zantiks LT kit

Overall unit size (external, approx. mm): 560 width, 410 depth, 870 height

Cage size for rodents (internal, mm): 360 width, 270 depth, 300 height

Tank sizes for aquatic (internal, mm) to maximum of: 360 width, 270 depth, 300 height

Technical specification

Integrated unit Built-in stimuli
computer lights (overhead)
software solid, liquid delivery (motor)
camera visual images (floor / ceiling screen)
experimental chamber

Experimental chamber inserts

Rodents Fish
Food hopper Arena tanks
5-aperture insert Dividing inserts
Y-maze light/dark inserts
Y-maze

Tanks / cages

The LT can accommodate many different sized tanks.

  • The largest tank/ cage that can fills the experimental chamber measures 27cm x 36cm x 30cm (W x L x H)
  • A maximum of four narrow tanks fit in the experimental chamber, these have opaque ends and clear sides and bottom; they measure 7.5cm x 36cm x 30cm (W x L x H)
  • The medium tanks are used for 3D tracking, there are made from infrared material with a clear base; they measure 13cm x 36cm x 20cm
  • The standard AD tank (which fills the AD experimental chamber) can be used in the LT chamber. The photo below helps demonstrate the relatively different sized tanks (and chambers) between the AD and LT units.

Opaque side inserts

To prevent singly-housed animals from seeing the animal/s in adjacent tanks, use the opaque side inserts that slot on to the outside of the tank walls using tabs, as illustrated below.

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Zantiks tanks pictured from the side, showing relative sizes. From the front back: AD tank (from the end), 3D tracking tank (LT; from the side), narrow LT tank (from the side), large LT tank (from the side)
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Zantiks tanks photographed from above, showing relative sizes. From the left hand side: large LT tank, narrow LT tank, 3D tracking tank (LT), AD tank
Lt Large Tank Opaque Side Thin Tab
Close up of LT large tank with thin tab attaching the opaque side
Lt Narrow Tank Opaque Side Tab
LT narrow tank with tabs attaching opaque sides
Lt Narrow Tanks Adjacent With Tab
LT narrow tanks clipped together with tabs
Lt Narrow Tank Adjacent Opaque Insert
LT narrow tanks adjacent, with opaque insert (blocking view between tanks)
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Adjacent LT narrow tanks (with clear sides enabling animals to see in to adjacent tank)
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Adjacent LT narrow tanks with opaque lengthways insert (blocking view between tanks)
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Side screen

An extra side screen can be bought as an add-on to the LT unit, which allows for lateral presentation of images against the back wall of the unit. The side screen plugs in to the unit and is operated in the same way as the screen underneath / over the tank. Only one screen can be used at a time.

To see a demo script demonstrating how the side screen works, click through to the Side screen demo page. Or, please email your enquiry to info@zantiks.com.

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Built-in stimuli

Solid and liquid delivery - both solid and liquid food reward, as well as chemical liquid stimuli (ie alarm substance) can be supplied when specified by the Zanscript script.

  • Visual stimuli - supplied on a screen from underneath the tank. These can be specified with sizes, shapes, colours, locations and bitmaps. As they are displayed via a screen, response times and update times can be controlled up to the 60Hz refresh rate of the display.
  • Overall illumination - supplied from above the tank. This can be used to provide ambient lighting, circadian rhythm and/or light flashes to elicit startle responses. Colour brightness and duration can be specified with ms accuracy.
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How to operate

The Zantiks LT is simple to operate. Fully networked, Zantiks units are supplied with a router and controlled via a web browser, allowing users to operate them from their own phone, tablet, laptop, PC, Mac or Chromebook. From the web browser on your device you can edit scripts, start and stop chosen services, view live video, download results (.csv files).

To operate a Zantiks unit connect to the same network as the unit and open up the home page of the Zantiks unit, the Zantiks Control Console, by typing in the IP address of the unit in the browser (this is displayed on the LT's screen when you boot up). From this home page you can operate all your networked units.

For details on how to use the Zantiks Control Console on your device, look at the Zantiks Control Console manual.

High-throughput studies are easy to conduct with multiple units. All units are operated via the same web browser and can run identical experiments consecutively.

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Tracking and video recording

The LT unit can track multiple animals that are housed singly in different arenas.

The scripts can be written to track the locomotion, distance travelled and time spent in different zones. They can also be written to record activity, such as a mouse's nose poke in a zone.

The video recording made below was taken of an eight-week old mouse in a Y-maze. The overlays in the video show, firstly that the unit was tracking the mouse in one arena (with a blue overlay), and secondly that there were four detector zones set up in the Y-maze, so that the mouse's movement between the arms of the Y-maze could be recorded.

Video recording of a Y-maze protocol with a mouse in the Zantiks LT unit